Applied Biology

Name some of the stationary phases of gas chromatography?

a) Cyanopropylphenyl dimethyl polysiloxane, b) Carbowax polyethylene-glycol, c) Biscyanopropyl cyanopropylphenyl polysiloxane d) Diphenyl dimethyl polysiloxane

Which of the following is not a detector of gas chromatography?

a) Electron capture detector b) Radium ion detector c) Flame ionization detector d) Thermal conductivity detector. Ans. b) Radium ion detector is not the…

Name the scientists who got noble prize for invention of chromatography?

Martin and Synge got noble prize in Chemistry in the year 1952 for the invention of partition chromatography. Their full names are Archer John…

Lecture-1 : Chromatography

Lecture-1 : Chromatography “Chroma” means colour and “graphy” means graphical representation. Separation of components of a mixture on the basis of colour and then identifying…

Lecture 3: Ion- Exchange chromatography

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Lecture 2: Gel filtration chromatography

Lecture 2: Bioanalytical tools Important points It is also called as molecular sieve chromatography or sieve exclusion chromatography. A column made up of porous…

Lecture 2: SEQUENCE INFORMATION RESOURCES

(BIOINFORMATICS) LECTURE 2- BT-501 With the launch of the Human Genome Project, enormous data came to be known to humans. And it was almost…

A mixture is having 5 components A, B, C, D and E of size 2, 4, 6, 8 and 10 µm. This mixture is applied to a column having beads of size, 2, 4, 6, and 8 µm. After this, mobile phase is applied. The value of Partition coefficients (Kd) of sample components in stationary and mobile phase will be in the ratio: a) E>D>C>B>A b) A>B>C>D>E c) D>C>B>A>E d) A>D>B>C>E

Correct Answer is b. Explanation: Distribution coefficient = (concentration of component in stationary phase)/ (concentration of component in mobile phase). It is represented by…

A protein domain a) cannot have beta sheets. b) can fold only in the presence of the rest of the protein. c) is always composed of multiple peptides. d) can be disordered.

Ans… a) is false. Protein domains can have beta barrel. b) is also false. Protein domains are autonomous structures and can fold independently. c)…